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Hillwilliam -> RE: Bin Laden..........DEAD (5/2/2011 11:02:04 AM)
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quote:
ORIGINAL: flcouple2009
quote:
ORIGINAL: Hillwilliam
DNA testing is done by extracting a sample and using DNA polymerase to create more of the molecules.
Step 2. Use a 'restriction enzyme' that recognizes certain sequences and cuts the DNA at those sequences. As everyone's DNA is different, the cuts will yield parts of different lengths.
Step 3. Place the mixture of DNA fragments on a semipermeable gel and place a small DC charge across the gel.
Step 4. The fragments will migrate thru the gel in reverse proportion to their Charge/Mass ratio, (the DNA has a lot of phosphate in it which has a negative charge)
Preliminary results can be obtained in 12-24 hours. If you need a fast quick and dirty, you slightly increase the DC voltage which makes the fragments move a bit faster.
How do I know?
I did it when I was in grad school.
There is also a test that takes 4 hours which is good enough for a preliminary match.
I had the link earlier but would have to find it again.
You are probably correct. what most folks don't realize is that most analytical techniques are invented by a bunch of eggheads in a university lab with a LOT of cool equipment. After the technique is perfected and vetted, it must then be 'dumbed down' so that a typical tech can do it without undue difficulty and still obtain good data. This typically means dwawing the process WAAAAAAAAAAAAAY out.
As for speeding things up, if I owned a DNA lab or an EPA certified water lab (or other private analytical facility), I couldn't afford to have my analysts shut the production line down for a coupla months while my employees worked out a protocol that might or might not save time in the future.
Let the guys in the uni do the hard work and then I'll adapt.
I hope the above coupla posts helps those folks wondering how OBL's DNA was analyzed so quickly.
If you need more info, My mailbox is open on the other side.
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